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Stem cell research and through that cell therapy have become increasingly popular research topic as cell therapy can cure many diseases and conditions that in the past has been beyond imagination. Stem cells have an ability to differentiate and self-renew. Those abilities can be harnessed for many different therapeutic applications. Stem cells are classified into different types depending on their differentiation potency. The types are in descending potency order: totipotent, pluripotent, multipotent, oligopotent and unipotent. (1) Two of these are interesting for the regeneration therapy point of view as they are available for developing regenerative therapy.

Method tips for iPSC:

Efficient reprogamming of fibroblasts, PBMCs and cd34+ cells can be done with Nucleofector™ technology and

L7™ PBMC reprogramming bundle  for generation of iPSC from mononuclear cells and L7 hPSC culture system, feeding is required only every second day!

Pluripotent stem cells (PSCs)

  • Originate from all germ layers of the embryo, embryonic stem cells (ESCs)
  • Induced pluripotent stem cells (iPSCs) are artificially generated from somatic cells by reprogramming the cells gene expression patterns. Reprogramming can be done with gene editing tools like CRISPR-Cas9 and transfection methods like Nucleofector™.
  • Start to differentiate to different multipotent stem cells
  • Used in lot of regenerative medicine and research! See the tips on the left side

Multipotent stem cells

  • Specialized to differentiate to certain lineage in a narrowed spectrum (oligopotent stem cells)
  • Example: Hematopoietic stem cells, differentiate to all types of blood cells
  • Adult stem cells and compatible culturing and differentiation media: mesenchymal cells (PT-2501), adipose-derived (PT-5006), dental pulp stem cells (PT-5025), hematopoietic stem cells (cd34+ cells, several options, contact us for recommendation).

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1. Zakrzewski W, Dobrzyński M, Szymonowicz M, Rybak Z. Stem cells: past, present, and future. Stem Cell Res Ther. 2019 Feb 26;10(1):68. doi: 10.1186/s13287-019-1165-5. PMID: 30808416; PMCID: PMC6390367.