BioNordika News

Troubleshooting Protein expression, Part 3
Cell-free protein expression, PURE joy! The previous post of this series hinted at an efficient way of getting around problems with in vivo protein expression; to use a cell-free, in vitro, expression system. Cell-free protein expression offers a way to minimize the...

Free samples from New England Biolabs
NEB offers a wide variety of high quality solutions covering for example a whole range from nucleic acid purification, PCR amplification to DNA assembly/cloning, as well as NGS library preparation. NEBNext® - Next Generation Sequencing Library Preparation NEB offers...

Tips for investigating cell death (Part 1)
Wanted dead or alive – Tips for investigating cell death (Part 1) – autophagy and apoptosisCell death research has become an intensive research topic as disturbance in the natural cell death process is contributing to several different diseases ranging from...

Troubleshooting protein expression, Part 2
Continuing our look at strategies for handling expression of troublesome proteins, this post is the second part in our a series on protein expression, purification, crystallization, and analysis. If you missed the first part, you can find it here. In this brief...

Quick guide to get started with IHC double staining
Quick guide to get started with immunohistochemistry double staining – ImmPRESS Duet Double staining polymer kit (Vector) Staining the same tissue for two different targets at the same time is not only a great way to increase the information you get from the...

RNA-Seq – A powerful tool for complex diseases and cancer research
RNA-seq (RNA sequencing) has become a powerful method for transcriptome analysis. Using next-generation sequencing (NGS) it is widely used for gene expression analysis, including detection of mutations, fusion transcripts, alternative splicing, and...

Fast and easy way to test for mycoplasma
Mycoplasma is common bacteria, that can easily infect cell cultures and cause difference in their behavior. It’s important to not only test new cells, but also do regular testing of ongoing cell cultures. Mycoplasma testing is a good scientific practice, and it is...

Pluripotent and multipotent stem cells and available research tools
Stem cell research and through that cell therapy have become increasingly popular research topic as cell therapy can cure many diseases and conditions that in the past has been beyond imagination. Stem cells have an ability to differentiate and self-renew. Those...

Having trouble when amplifying GC-rich sequences? Here are four tips on how to troubleshoot
What is the meaning of GC-rich and why can it be challenging to amplify? A GC-rich template refers to a DNA sequence where 60% (or greater) of the bases present are G (guanine) or C (cytosine). Only 3% of the human genome is GC-rich, but these regions are often found...

3 tips for Cell Culture work
We are often so used to the “routine work” in cell culture room, that we forget to even consider that changes in routines and trying out new methods could save us a lot of time and create more flexibility to lab work. Check out our tips to see if you could take...

Troubleshooting Protein Expression
Part 1: Improving protein yield using T7 Express and NEB Express E.coli expression systems Successful protein expression and isolation are prerequisite for many downstream applications, such as protein crystallization and functional assays. While there exists a myriad...

All astrocytes are not the same – location matters!
Astrocytes have important role in the brain, for instance in controlling neurogenesis, blood-brain barrier permeability, neuroinflammation and maintaining homeostasis (1). Astrocytes are also very different depending on which area of the brain they are located. Here...

Say Goodbye to Manual Gels: Automated DNA size selection has arrived!
Automated size selection is a superior alternative to manual gel extraction, allowing scientists to save time and money and improve the efficiency of sequencing runs and analysis. Many scientists use agarose gels as a manual step to size-select their libraries prior...

Simplifying the search for protein crystals
Discovering the optimal conditions to crystallize your favourite protein can be a tedious, time-consuming, and expensive process. Multiple variables such as salinity, pH, temperature, the presence of ligands, and more can all affect the end result. The use of a...

Sugarcoated proteins on cancer cells and lectins as a tool to target them
Glycobiology plays an important role in oncology. Most cancer researchers have thought about the genome and mutations in their research topic, and glanced through papers of proteomics, but the third major biomolecule group, carbohydrates, is often getting less...

Logos Biosystems automated cell counters
Count cells like a boss Cell counting is a fundamental quality control procedure in research. It’s simple, but it’s also error-prone, time-consuming, and highly subjective. BioNordika is now proudly representing Logos Biosystems automated cell counters of the LUNA™...

How to rescue cells after mycoplasma contamination?
To find out information how to detect mycoplasma contamination read our other post: https://www.bionordika.se/detecting-mycoplasma/ Mycoplasma contamination is so common that at some point all cell culture laboratories will have to deal with it. When the cells are...

Synthesis of high-quality RNA
The power of vitro transcribed (IVT) mRNA The power of IVT mRNA technology became aware again with the latest development of covid-19 vaccines. Messenger RNA (mRNA) vaccine is a type of vaccine that uses mRNA molecules, which encode disease-specific antigens to...

Strange results in cell experiments? Could it be mycoplasma?
Did you know that almost up to 35% of continuous cell lines have mycoplasma contamination according to an article where mycoplasma incidence was compared in different cell lines? Mycoplasma is not only a problem in old cell lines, but it can be present in new cell...

Webinar: Breast Cancer recurrence risk and subtyping
Welcome to BioNordika & HTG Molecular special webinar on International Women's Day! Special session with Durviz: "Breast Cancer Recurrence Risk Classification and Molecular Subtyping using HTG EdgeSeq Technology" - Welcome session- Introduction: HTG Molecular...
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