DNA methylation and histone modification are referred to as epigenetic modifications. Global changes in the epigenetic landscape along with genetic alterations are a hallmark of cancer. To understand the cause of changes in the epigenetic landscape, researchers are using sophisticated tools to analyze epigenetic deviations in cancer versus normal cells. We at BioNordika would like to emphasize some of these tools to further support your cancer research and provide you with innovative ideas.
BioNordika offers a broad product line and number of tools for the analysis of DNA modifications and DNA-associated proteins. We offer kits for the enrichment of methylated DNA, to study different methylation states, bisulfite conversion and nucleosome assembly. We also offer a wide range of restriction enzymes that can be used to analyze DNA methylation. Moreover, we offer solutions for epigenetic analysis such as ChIP-grade antibodies and sequencing kits for methylome analysis.
Please find a selection of epigenetic tools from our suppliers below. If you don’t find what you are looking for please contact us at firstname.lastname@example.org.
New England Biolabs
NEBNext Enzymatic Methyl-seq (E7120S/L)
The Kit provides a high-performance enzyme-based alternative to bisulfite conversion for methylome analysis. The consistently high conversion performance and minimized DNA damage with the EM-seq™ protocol, in combination with highly efficient NEBNext Ultra II library prep, result in superior detection of CpGs with fewer sequencing reads.
NEBNext® Q5U® Master Mix (E0597S/L)
NEBNext® Q5U is a modified version of Q5® High Fidelity DNA Polymerase, optimized for amplification of uracil-containing NGS libraries. This hot start formulation is designed to read through uracils, providing robust, high-fidelity, and uniform library amplification. NEBNext Q5U Master Mix is also compatible with library amplification in bisulfite sequencing workflows.
EpiMark® 5-hmC and 5-mC Analysis Kit (E3317S)
This kit can be used to analyze and quantitate 5-methylcytosine and 5-hydroxymethylcytosine within a specific locus. The kit distinguishes 5-mC from 5-hmC by the addition of glucose to the hydroxyl group of 5-hmC via an enzymatic reaction utilizing T4 β-glucosyltransferase (T4-BGT). When 5-hmC occurs in the context of CCGG, this modification converts a cleavable MspI site to a noncleavable one.
Human Sirtuin 1, recombinant (Cat. Nr. AS-72212)
The recombinant human Sirtuin 1 (GenBank Accession #: NM_012238) with 193- 741 amino acids and GST tag at its N-terminal was expressed in E. coli. Its activity can be measured using enzymatic assay with a fluorogenic or FRET substrates. Sirtuins are histone deacetylases (HDACs) that act as transcriptional repressors of genes catalyzing the removal of acetyl groups from a ε-N-acetyl lysine of histone.
SensoLyte® 520 FRET Sirt1 Assay Kit Fluorimetric (Cat. Nr. AS-72115)
The SensoLyte® 520 FRET SIRT1 Assay Kit provides a convenient, two-step homogeneous procedure for measuring sirtuin 1 activity using a FRET substrate. (contact us for more information, more peptides, recombinant proteins, and SensoLyte Assay kits are available)
Histone deacetylase, HDAC substrate (Cat. Nr. AS-61855)
A fluorogenic substrate to characterize the HDAC activity.
Epigenetics Screening Library (96-Well) (Cat. Nr. 11076)
The kit contains more than 140 small molecules that are known to modulate the activity of a variety of epigenetic ‘writers and erasers’ and ‘reader’ proteins in a 96-well Matrix tube rack format as 10 mM stocks in DMSO. It includes compounds that modulate the activity of methyltransferases, demethylases, HATs, HDACs, and acetylated lysine reader proteins.
Diagenode offers solutions for epigenetics research, such as the innovative Bioruptor and Megaruptor shearing instruments, reagent kits and high-quality antibodies to streamline DNA methylation, ChIP, and ChIP-seq workflows.
Cell Signaling Technology
CUT&RUN Assay Kit (#86652)*
CUT&RUN pAG-MNase and Spike-In DNA (#40366)
Cleavage Under Target & Release Using Nuclease (CUT&RUN) is a new technology launched by Cell Signaling Technology. It is a low cell number alternative to ChIP-qPCR and ChIP-seq to analyze protein-DNA interaction in chromatin. Some key benefits are: low cell input (only 100K required), results within 1-2 days, only 3-5 million high quality sequencing reads needed, compatible with rabbit and mouse antibodies and either qPCR and sequencing data can be achieved for histones, histone modifications, transcription factors, and cofactors. To ensure reproducible results a spike in control DNA is used to normalize signal between samples.
*The components in the kit can also be bought separately.
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