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Don’t get lost in the noise – Reveal true immunofluorescence. Diminish unwanted autofluorescence from non-lipofuscin sources even in the most challenging tissues.


Immunofluorescence (IF) utilizes antibodies labeled with a fluorescent molecule to localize and visualize proteins, glycans and small molecules in cells and tissues. IF is a great tool for scientists to study biological events. However, a key limitation of IF is the autofluorescence from intrinsic biomolecules.
Tissue autofluorescence often occurs from aldehyde fixation or from inherent native tissue components (such as collagen, elastin and red blood cells). The extent and intensity of autofluorescence background frequently make it difficult or impossible to distinguish specific signals from background especially in green and red channel fluorophores.

Historically, Sudan Black or similar dyes has been used to tackle the autofluorescence challenges. Sudan Black is especially good at lowering autofluorescence due to lipofuscin (yellow-brown pigment granules composed of lipid-containing residues of lysosomal digestion). However, Sudan Black is much less effective at lowering the fluorescence due to aldehyde fixation, connective tissue elements and red blood cells.

Vector laboratories has developed a TrueVIEW Autofluorescence Quenching Kit which provides a novel way to diminish unwanted autofluorescence from non-lipofuscin sources and dramatically improve signal-to-noise ratio. The quenching action of the kit reagents provides a clear, unambiguous, “true view” localization of the target antigen.