Gene expression is the process by which genetic information stored into a nucleic acid sequence is converted into a polypeptide chain, the building blocks of proteins. By transfecting engineered plasmid DNA, scientists routinely study the function of a protein of interest and/or its role in a signaling pathway.
It is essential for biologists to work with physiologically relevant cell models. However, several types of cells (primary cells, stem cells or cancer cell lines) remain difficult to transfect with DNA for different reasons: slow-dividing rates, cell fragility and cellular defense mechanisms. To address the current limits of DNA transfection Polyplus engineered an innovative delivery nanoparticle: jetOPTIMUS.
jetOPTIMUS is developed to improve DNA transfection efficiency in easy- and difficult-to-transfect cells. jetOPTIMUS improves cellular uptake and endosomal escape of DNA in adherent cells (even in hard-to-transfect cells) resulting in higher transfection efficiency. And by using minimum DNA quantity and reagent volume you are able to keep an excellent cell viability and morphology
jetOPTIMUS DNA transfection reagent
- Highly efficient: reach maximal gene expression in hard-to-transfect cells
- Cost-effective: use minimum reagent volume and DNA quantity
- Less toxic: keep an excellent cell viability & morphology
- Timesaving: optimized and ready-to-use protocol
jetOPTIMUS is a powerful transfection reagent that improves cellular uptake and endosomal escape of DNA in adherent cells (even in hard-to-transfect cells) resulting in higher transfection efficiency.
Transfection with jetOPTIMUS requires a minimum DNA quantity and reagent volume to keep an excellent cell viability and morphology.
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