Customized depletion of abundant RNAs

RNA-sequencing (RNA-Seq) is a high-throughput sequencing method that facilitates the study of the entire transcriptome. However, revealing the presence and quantity of the RNAs of interest in a biological sample can be difficult due to the abundance of highly expressed transcripts, such as ribosomal RNA (rRNA) that mask detection of low-abundance transcripts.

To overcome the challenge pre-designed kits are available for some sample types (e.g. human, mouse, rat, some bacteria) for the removal of highly abundant molecules prior to RNA-seq (Figure to the left presents NEBNext rRNA Depletion Workflow). However, due to the limited availability of pre-designed RNA depletion kits, this has been a limiting factor for scientists working with a wide variety of organisms. To cope with the problem, New England Biolabs is now providing a kit for customized depletion of unwanted RNAs. The NEBNext RNA Depletion Core Reagent Set (E7865) provides a customized approach to deplete unwanted RNAs from any organism and subsequently allows for accurate quantification of lowly expressed RNA species. Together with customized probes the NEBNext RNA Depletion Core Reagent kit is suitable for depletion of low- and high-quality RNA.