Immunofluorescence (IF) utilizes antibodies labeled with a fluorescent molecule to localize and visualize proteins, glycans and small molecules in cells and tissues. IF is a great tool for scientists to study biological events. However, a key limitation of IF is the...
Cell-free protein expression, PURE joy! The previous post of this series hinted at an efficient way of getting around problems with in vivo protein expression; to use a cell-free, in vitro, expression system. Cell-free protein expression offers a way to minimize the...
Wanted dead or alive – Tips for investigating cell death (Part 1) – autophagy and apoptosis Cell death research has become an intensive research topic as disturbance in the natural cell death process is contributing to several different diseases ranging from...
Continuing our look at strategies for handling expression of troublesome proteins, this post is the second part in our a series on protein expression, purification, crystallization, and analysis. If you missed the first part, you can find it here. In this brief...
Quick guide to get started with immunohistochemistry double staining – ImmPRESS Duet Double staining polymer kit (Vector) Staining the same tissue for two different targets at the same time is not only a great way to increase the information you get from the...
RNA-seq (RNA sequencing) has become a powerful method for transcriptome analysis. Using next-generation sequencing (NGS) it is widely used for gene expression analysis, including detection of mutations, fusion transcripts, alternative splicing, and...
Mycoplasma is common bacteria, that can easily infect cell cultures and cause difference in their behavior. It’s important to not only test new cells, but also do regular testing of ongoing cell cultures. Mycoplasma testing is a good scientific practice, and it is...
Stem cell research and through that cell therapy have become increasingly popular research topic as cell therapy can cure many diseases and conditions that in the past has been beyond imagination. Stem cells have an ability to differentiate and self-renew. Those...
What is the meaning of GC-rich and why can it be challenging to amplify? A GC-rich template refers to a DNA sequence where 60% (or greater) of the bases present are G (guanine) or C (cytosine). Only 3% of the human genome is GC-rich, but these regions are often found...
We are often so used to the “routine work” in cell culture room, that we forget to even consider that changes in routines and trying out new methods could save us a lot of time and create more flexibility to lab work. Check out our tips to see if you could take...
Part 1: Improving protein yield using T7 Express and NEB Express E.coli expression systems Successful protein expression and isolation are prerequisite for many downstream applications, such as protein crystallization and functional assays. While there exists a myriad...
Astrocytes have important role in the brain, for instance in controlling neurogenesis, blood-brain barrier permeability, neuroinflammation and maintaining homeostasis (1). Astrocytes are also very different depending on which area of the brain they are located. Here...
Automated size selection is a superior alternative to manual gel extraction, allowing scientists to save time and money and improve the efficiency of sequencing runs and analysis. Many scientists use agarose gels as a manual step to size-select their libraries prior...
Discovering the optimal conditions to crystallize your favourite protein can be a tedious, time-consuming, and expensive process. Multiple variables such as salinity, pH, temperature, the presence of ligands, and more can all affect the end result. The use of a...
Glycobiology plays an important role in oncology. Most cancer researchers have thought about the genome and mutations in their research topic, and glanced through papers of proteomics, but the third major biomolecule group, carbohydrates, is often getting less...
Count cells like a boss Cell counting is a fundamental quality control procedure in research. It’s simple, but it’s also error-prone, time-consuming, and highly subjective. BioNordika is now proudly representing Logos Biosystems automated cell counters of the LUNA™...
To find out information how to detect mycoplasma contamination read our other post: https://www.bionordika.se/detecting-mycoplasma/ Mycoplasma contamination is so common that at some point all cell culture laboratories will have to deal with it. When the cells are...
The power of vitro transcribed (IVT) mRNA The power of IVT mRNA technology became aware again with the latest development of covid-19 vaccines. Messenger RNA (mRNA) vaccine is a type of vaccine that uses mRNA molecules, which encode disease-specific antigens to...
Did you know that almost up to 35% of continuous cell lines have mycoplasma contamination according to an article where mycoplasma incidence was compared in different cell lines? Mycoplasma is not only a problem in old cell lines, but it can be present in new cell...
Date: 17th November 2021Time: 15.00-15:45 CET Title: Advancing life science research by refining transfection: strategies and tips Welcome to our webinar on Transfection tips with our partner Polyplus. Transfection is an indispensable tool for studies conducted...
