Welcome to join our NGS lunch seminar! Free sandwiches & coffee will be served at 12 before the seminar starts. Date and time: May 11th 2023 between 12-13:30Venue: Louis, Widerströmska huset, Tomtebodavägen 18a, Solna, Entrance level Widerströmska huset (just...
Cell-free protein expression, PURE joy! The previous post of this series hinted at an efficient way of getting around problems with in vivo protein expression; to use a cell-free, in vitro, expression system. Cell-free protein expression offers a way to minimize the...
NEB offers a wide variety of high quality solutions covering for example a whole range from nucleic acid purification, PCR amplification to DNA assembly/cloning, as well as NGS library preparation. NEBNext® – Next Generation Sequencing Library Preparation NEB...
Continuing our look at strategies for handling expression of troublesome proteins, this post is the second part in our a series on protein expression, purification, crystallization, and analysis. If you missed the first part, you can find it here. In this brief...
RNA-seq (RNA sequencing) has become a powerful method for transcriptome analysis. Using next-generation sequencing (NGS) it is widely used for gene expression analysis, including detection of mutations, fusion transcripts, alternative splicing, and...
What is the meaning of GC-rich and why can it be challenging to amplify? A GC-rich template refers to a DNA sequence where 60% (or greater) of the bases present are G (guanine) or C (cytosine). Only 3% of the human genome is GC-rich, but these regions are often found...
The power of vitro transcribed (IVT) mRNA The power of IVT mRNA technology became aware again with the latest development of covid-19 vaccines. Messenger RNA (mRNA) vaccine is a type of vaccine that uses mRNA molecules, which encode disease-specific antigens to...
Do you know what these scientists have in common who say ‘’it is working brilliantly’’, ‘’the reaction worked beautifully’’, ‘’more product’’, ‘’I am surprised by the result’’? Well, they all use NEB’s Q5 High-Fidelity DNA polymerase for target amplification. Q5...
