Tel 08-30 60 10 info@bionordika.se
BioNordika NGS Lunch Seminar May 11th 2023

BioNordika NGS Lunch Seminar May 11th 2023

Welcome to join our NGS lunch seminar! Free sandwiches & coffee will be served at 12 before the seminar starts. Date and time: May 11th 2023 between 12-13:30Venue: Louis,  Widerströmska huset, Tomtebodavägen 18a, Solna, Entrance level Widerströmska huset (just...
The common causes of autofluorescence and how to tackle them

The common causes of autofluorescence and how to tackle them

Immunofluorescence (IF) utilizes antibodies labeled with a fluorescent molecule to localize and visualize proteins, glycans and small molecules in cells and tissues. IF is a great tool for scientists to study biological events. However, a key limitation of IF is the...
Troubleshooting Protein expression, Part 3

Troubleshooting Protein expression, Part 3

Cell-free protein expression, PURE joy!  The previous post of this series hinted at an efficient way of getting around problems with in vivo protein expression; to use a cell-free, in vitro, expression system. Cell-free protein expression offers a way to minimize the...
Free samples from New England Biolabs

Free samples from New England Biolabs

NEB offers a wide variety of high quality solutions covering for example a whole range from nucleic acid purification, PCR amplification to DNA assembly/cloning, as well as NGS library preparation.   NEBNext® – Next Generation Sequencing Library Preparation NEB...
Tips for investigating cell death (Part 1)

Tips for investigating cell death (Part 1)

Wanted dead or alive – Tips for investigating cell death (Part 1) – autophagy and apoptosis Cell death research has become an intensive research topic as disturbance in the natural cell death process is contributing to several different diseases ranging from...
Troubleshooting protein expression, Part 2

Troubleshooting protein expression, Part 2

Continuing our look at strategies for handling expression of troublesome proteins, this post is the second part in our a series on protein expression, purification, crystallization, and analysis. If you missed the first part, you can find it here. In this brief...
Quick guide to get started with IHC double staining

Quick guide to get started with IHC double staining

Quick guide to get started with immunohistochemistry double staining – ImmPRESS Duet Double staining polymer kit (Vector) Staining the same tissue for two different targets at the same time is not only a great way to increase the information you get from the...
Fast and easy way to test for mycoplasma

Fast and easy way to test for mycoplasma

Mycoplasma is common bacteria, that can easily infect cell cultures and cause difference in their behavior. It’s important to not only test new cells, but also do regular testing of ongoing cell cultures. Mycoplasma testing is a good scientific practice, and it is...
3 tips for Cell Culture work

3 tips for Cell Culture work

  We are often so used to the “routine work” in cell culture room, that we forget to even consider that changes in routines and trying out new methods could save us a lot of time and create more flexibility to lab work. Check out our tips to see if you could take...
Troubleshooting Protein Expression

Troubleshooting Protein Expression

Part 1: Improving protein yield using T7 Express and NEB Express E.coli expression systems Successful protein expression and isolation are prerequisite for many downstream applications, such as protein crystallization and functional assays. While there exists a myriad...
All astrocytes are not the same – location matters!

All astrocytes are not the same – location matters!

Astrocytes have important role in the brain, for instance in controlling neurogenesis, blood-brain barrier permeability, neuroinflammation and maintaining homeostasis (1). Astrocytes are also very different depending on which area of the brain they are located. Here...
Simplifying the search for protein crystals

Simplifying the search for protein crystals

Discovering the optimal conditions to crystallize your favourite protein can be a tedious, time-consuming, and expensive process. Multiple variables such as salinity, pH, temperature, the presence of ligands, and more can all affect the end result. The use of a...
Logos Biosystems automated cell counters

Logos Biosystems automated cell counters

Count cells like a boss Cell counting is a fundamental quality control procedure in research. It’s simple, but it’s also error-prone, time-consuming, and highly subjective. BioNordika is now proudly representing Logos Biosystems automated cell counters of the LUNA™...
How to rescue cells after mycoplasma contamination?

How to rescue cells after mycoplasma contamination?

To find out information how to detect mycoplasma contamination read our other post: https://www.bionordika.se/detecting-mycoplasma/ Mycoplasma contamination is so common that at some point all cell culture laboratories will have to deal with it. When the cells are...
Synthesis of high-quality RNA

Synthesis of high-quality RNA

The power of vitro transcribed (IVT) mRNA The power of IVT mRNA technology became aware again with the latest development of covid-19 vaccines. Messenger RNA (mRNA) vaccine is a type of vaccine that uses mRNA molecules, which encode disease-specific antigens to...