Wanted dead or alive – Tips for investigating cell death (Part 2) – Necrosis, necroptosis, pyroptosis The second part of the post series introduces three pro-inflammatory forms of cell death: necrosis, necroptosis and pyroptosis. The first part of the article series...
Multiplexing, a powerful technique that allows the visualization of multiple biomarkers within a single sample, has revolutionized the field by offering researchers valuable insights into the spatial context of complex cellular processes and interactions. By analyzing...
In this post we will continue our series highlighting tools for protein expression, purification, and analysis. The time has come to look at isolating your expressed protein. If you have missed our previous chapters on protein expression, you can find them here. ...
Getting to know your neurons and synapses more in depth? We got you covered! Read more about the different products from Biotium including CF® Dye labeled dextrans, nerve terminal dyes like SynaptoGreen and SynaptoRed, ion indicator dyes like tetra- and penta- cesium,...
The development of mRNA (messenger RNA) vaccines, namely the COVID-19 vaccines has rekindled the interest in methodologies for RNA production via In Vitro transcription (IVT). mRNA vaccines use mRNA molecules, which encode disease-specific antigens to trigger an...
Welcome to BioNordika’s Instrument showroom at BioMedicum! Date and time: 8th of June between 12.00-15.00 Venue: Biomedicum, Karolinska Institutet Room: Peter Reichard Entrance level Biomedicum.Address: Solnavägen 9, Solna Come and explore...
Welcome to join our NGS lunch seminar! Free sandwiches & coffee will be served at 12 before the seminar starts. Date and time: May 11th 2023 between 12-13:30Venue: Louis, Widerströmska huset, Tomtebodavägen 18a, Solna, Entrance level Widerströmska huset (just...
Immunofluorescence (IF) utilizes antibodies labeled with a fluorescent molecule to localize and visualize proteins, glycans and small molecules in cells and tissues. IF is a great tool for scientists to study biological events. However, a key limitation of IF is the...
Cell-free protein expression, PURE joy! The previous post of this series hinted at an efficient way of getting around problems with in vivo protein expression; to use a cell-free, in vitro, expression system. Cell-free protein expression offers a way to minimize the...
NEB offers a wide variety of high quality solutions covering for example a whole range from nucleic acid purification, PCR amplification to DNA assembly/cloning, as well as NGS library preparation. NEBNext® – Next Generation Sequencing Library Preparation NEB...
Wanted dead or alive – Tips for investigating cell death (Part 1) – autophagy and apoptosis Cell death research has become an intensive research topic as disturbance in the natural cell death process is contributing to several different diseases ranging from...
Continuing our look at strategies for handling expression of troublesome proteins, this post is the second part in our a series on protein expression, purification, crystallization, and analysis. If you missed the first part, you can find it here. In this brief...
Quick guide to get started with immunohistochemistry double staining – ImmPRESS Duet Double staining polymer kit (Vector) Staining the same tissue for two different targets at the same time is not only a great way to increase the information you get from the...
RNA-seq (RNA sequencing) has become a powerful method for transcriptome analysis. Using next-generation sequencing (NGS) it is widely used for gene expression analysis, including detection of mutations, fusion transcripts, alternative splicing, and...
Mycoplasma is common bacteria, that can easily infect cell cultures and cause difference in their behavior. It’s important to not only test new cells, but also do regular testing of ongoing cell cultures. Mycoplasma testing is a good scientific practice, and it is...
Stem cell research and through that cell therapy have become increasingly popular research topic as cell therapy can cure many diseases and conditions that in the past has been beyond imagination. Stem cells have an ability to differentiate and self-renew. Those...
What is the meaning of GC-rich and why can it be challenging to amplify? A GC-rich template refers to a DNA sequence where 60% (or greater) of the bases present are G (guanine) or C (cytosine). Only 3% of the human genome is GC-rich, but these regions are often found...
We are often so used to the “routine work” in cell culture room, that we forget to even consider that changes in routines and trying out new methods could save us a lot of time and create more flexibility to lab work. Check out our tips to see if you could take...
Part 1: Improving protein yield using T7 Express and NEB Express E.coli expression systems Successful protein expression and isolation are prerequisite for many downstream applications, such as protein crystallization and functional assays. While there exists a myriad...
Astrocytes have important role in the brain, for instance in controlling neurogenesis, blood-brain barrier permeability, neuroinflammation and maintaining homeostasis (1). Astrocytes are also very different depending on which area of the brain they are located. Here...
