RAFT™ 3D Cell Cultures
Immunoflourescence Visualization of Fibroblast or Cancer Cells in RAFT™ 3D Cell Cultures
This technical note shows that immunofluorescence can be performed easily and routinely on RAFT™ 3D Cell Cultures.
Cells are embedded in collagen I and RAFT™ Absorbers are utilized to condense and create well-controlled, high-density collagen scaffolds with cell type(s) of choice. This represents a natural, more in-vivo environment for cells to grow. RAFT™ is a simple-to-use, semi-automated system that creates 3-dimensional cell cultures that are easy to handle.
hiPSC generation and hPSC culture
Lonza L7™ hiPSC Reprogramming and hPSC Culture System – A Case Study
In this iPSC reprogramming case study PBMCs are reprogrammed to generate hiPSCs. The L7™ hiPSC Reprogramming and hPSC Culture System from Lonza offers a complete and xeno-free system for the generation of human induced pluripotent stem cells (iPSCs), and the maintenance and expansion of human iPSCs.
Next Generation Sequencing
Low input ChIP-Seq using the NEBNext® Ultra™ DNA Library Prep Kit for Illumina®
The NEBNext Ultra DNA Library Prep Kit uses novel reagents and a quick, easy workflow, to generate ChIP-Seq libraries from sub-nanogram quantities of ChIP DNA generated with theSimpleChIP Enzymatic Chromatin IP Kit from Cell Signaling Technology.