We are often so used to the “routine work” in cell culture room, that we forget to even consider that changes in routines and trying out new methods could save us a lot of time and create more flexibility to lab work. Check out our tips to see if you could take advantage of them.
1. Frozen blood and immune cells
Working with primary cells is exciting as they can offer more biologically relevant information in comparison to cell lines, but often means long days and inconvenient working hours as you can’t always control what time (or even day) your samples or tissue will arrive, and, thus, when you can start processing. Much of the laborious and inconvenient work can be avoided by using frozen primary cells instead.
BioNordika’s partner Lonza offers a selection of blood cells, like PBMCs and immune cells that have already been isolated and sorted, so that you can just thaw a vial and start your experiment when it suits you best. The cell vials guarantee a number of viable cells making planning experiments easier. Each sample has already been tested for hepatitis, HIV and mycoplasma, meaning that you can work without concerns.
To see if your cell of interest is available, contact info@bionordika.se
P.S: In addition to blood and immune cells, primary cells are also available from different tissues, like, liver, intestines or lungs.
2. Automated Cell counting
Cell counting is a fundamental quality control procedure in research. It is straightforward, but manual counting can also be error-prone, time-consuming, and highly subjective. BioNordika now proudly represents Logos Biosystems, and their line of automated cell counters of the LUNA™ family.
The LUNA™ family instruments offer cell counters for all research purposes. The instruments are very easy to use and provide high efficiency cell countingwith less time required for each count.
LUNA™ cell counters are even able to identify live cells, dead cells, cell density, clusters of cells, and cell size.
For a free demo of Luna cell counter, contact Helene.wahlstedt@bionordika.se
And for more information, check out our previous blog post on Luna Cell Counters.
Luna-FL Dual Fluorescence Cell Counter is an example of easy-to-use automated counter that can be purchased through BioNordika
3. Scaling up the cell culture using CELLdisc
When working with larger cell cultures or with experiments that could eventually be scaled up for pre-GMP production we recommend using CELLdisc (Greiner), instead of normal culturing flasks or plates.
CELLdisc consists of several layers of coated plastic, greatly increasing the tissue culture area, so that media can be changed easily to all layers. Cell discs are available in different sizes (1-40 layers, 250-10000cm2), and with different coatings to suit your needs. They can also be combined with filtered venting caps and tubes to help in removal of media.
To order a free sample contact info@bionordika.se
4. Extra: Don’t waste time on mycoplasma infected cells!
Read our previous blog post on mycoplasma detection.
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